List of publications on a keyword: «электрофорез»


Биология

Publication date: 11.09.2019
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Pokhilenko Viktor Danilovich , doctor of technical sciences , leading research scientist
Perelygin Vladimir Vladimirovich , candidate of biological sciences , leading research scientist
Kalmantaev Timur Akhmerovich , candidate of biological sciences , research scientist
Detushev Konstantin Vladimirovich , degree-seeking student , research scientist
Chukina Irina Anatolevna , degree-seeking student , engineer
FBIS «State Research Center for Applied Microbiology and Biotechnology» of Rospotrebnadzor , Московская обл

«Antimicrobial properties of the peptide substance Bacillus Subtilis PSF-19»

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The subject of the study is a strain of Bacillus subtilis PSF-19 isolated by us from vegetable raw materials (Passiflora preparation), which is capable of producing antimicrobial substance (AMV) suppressing pathogenic bacteria. The article discusses the method and conditions of extraction from the culture fluid fraction of AMV, active against Listeria monocytogenes – one of the dangerous intestinal pathogens that infect food. Using biochemical methods and mass spectroscopy, the molecular weight and the peptide nature of the active fraction of AMV were determined. A fraction of AMV with a molecular weight of 3,4–3,6 kDa has bactericidal activity, which is destroyed by treatment with proteolytic enzymes, which allowed it to be attributed to the group of low-molecular antimicrobial peptides – bacteriocins. The studies allow to consider the strain Bacillus subtilis PSF-19 as a producer of bacteriocin, especially effective against pathogens of intestinal listeriosis. The establishment of the fact of destructibility of proteolytic enzymes, finding the conditions of microbiological synthesis of AMV, its isolation and accumulation for research, provide opportunities for practical use as a means for decontamination of the environment instead of traditional antibiotics.

Экология

Publication date: 21.02.2017
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Gabitova Aigul Aidarovna , candidate of biological sciences , доцент
FSBEI HE "Bashkir State University" , Башкортостан Респ
Yanbaev Ruslan Yulaevich , аспирант
Federal State Budgetary Educational Institution of Higher Education «Bashkir State Agrarian University» , Башкортостан Респ
Bakhtina Svetlana Yurievna , аспирант
FSBEI Ufa Institute of Biology RAS , Башкортостан Респ

«The differences in the structure of tree populations in different site-specific conditions»

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В статье приведены результаты изучения влияния различий в лесорастительных условиях на дифференциацию и полиморфизм популяций дуба черешчатого на Южном Урале, полученные с помощью электрофоретического анализа и аллозимных маркеров.